The Mosque of Kota Iskandar (Malay: Masjid Kota Iskandar) is a Johor state mosque located at Kota Iskandar, Iskandar Puteri, a Johor State Administrative Centre at Johor Bahru District, Johor, Malaysia. It is the second state mosque of Johor after Sultan Abu Bakar State Mosque in Johor Bahru. This modern futuristic mosque was officially opened on 16 June 2015 and was named after the late 24th and fourth sultan of Johor, Almarhum Sultan Iskandar ibni Almarhum Sultan Ismail.

The mosque design is being developed by the architect as a 'Garden Mosque', surrounded by Islamic gardens with water features, scented plants and shaded trees. The interplay of green spaces with water elements forms the prevailing urban fabric of the mosque ground. The outdoor landscaped area shall be suitable for praying spill over as well as large gatherings such as 'Maulidul Rasul' function.

An agreement was made between the State Government of Johor Darul Ta’zim, Johor State Secretary Incorporated and Cahaya Jauhar Sdn Bhd to develop a mosque at kota Iskandar.